I've successfully cloned my fragments into pGL3 (Luciferase) vector, and I want to see if the bands that I see on my agarose gel are correct by sequencing. So I was just wondering what the ideal concentrations of template / primer should be, and if there is any necessity of digestion of plasmid (In other words, can I sequence the whole plasmid, or should I cut the plasmid using RENs and isolate the insert by gel purification, then send for sequencing?)