6 Questions 4 Answers 0 Followers
Questions related from Donghyun Dave Lee
Is there any difference in performing ChIP on suspension cells vs. adherent cells? I'm currently using Activemotif ChIP-IT Express Enzymatic Kit
07 July 2016 5,821 4 View
I've successfully cloned my fragments into pGL3 (Luciferase) vector, and I want to see if the bands that I see on my agarose gel are correct by sequencing. So I was just wondering what the ideal...
11 November 2013 2,690 0 View
I'm trying to amplify using bisulfite converted dna template (to preserve methylation signature) following is my target region: (and i assume that cytosines that are non-cpg are converted to t...
08 August 2013 2,565 26 View
Is there a good place to request for gene synthesis service? and also sub-cloning into a vector of choice? Thank you!
07 July 2013 7,878 2 View
If possible, I would like some explanation about luciferase assay I'm trying to have several different portions of a specific gene promoter cloned into separate luciferase reporter vector...
07 July 2013 6,626 1 View
I'm wondering if there would be a way to join 2 PCR fragments together Probably won't work with Overlap PCR, and here's why: I want to see if there would be any effect by methylation of a gene...
07 July 2013 4,032 2 View
