I'm trying to fix a mouse model for AD cognitive research. It's suggested that APP/PS1dE9 model is suitable and commonly used for cognitive research. I'm not sure exactly what the reasons are since I have no experience in this field. Does anyone have experience with this model? and why is this model chosen for cognitive trails?
Cognitive tests with this model is often done on 9-12 month old animals, the time seems a bit late though some cognitive deficits can be detected at this stage. I'll probably have to plan all my experiments within 12 months. Not much information can be found on the strain description on Jax. I wonder when is the time for cognitive tests decided, for this seems highly consistent in different trails for the same model, such as 6 months and 12 months for 3xTg-AD
Also I've noticed reports on retinal A-beta amyloid dipositions in 12month old APP/PS1dE9 model, but no visual impairments were specified. How does this affect cognitive test involving visual cues (e.g. Morris Water Maze) if the tests are often carried out around this age for this model?
Any experience with this model would be greatly appreciated. Thanks
Hi Lin-
What does 'cognitive trails' mean? Do you mean trials?
There are many reasons for using different AD models - the one you should choose it the one that can answer the question you're trying to ask. For starters, a solid review on this topic was published earlier this year: http://www.ncbi.nlm.nih.gov/pubmed/24795750
When studying animal behavior (and, more importantly, correlating behavior to protein/gene expression), I feel it is best to include at least two age groups. Three is preferable. There is a large amount of data on the behavior and pathology of APP/PS1dE9 mice at various age groups. I would recommend reading the original paper from the Borchelt lab first http://www.ncbi.nlm.nih.gov/pubmed/14645205
I don't know much about retinal degeneration. I assume that, if no impairments were indicated, they weren't observed. If you choose this model, it may be best to answer this question yourself.
Hope this helps!
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