I've been searching for suitable amyloid beta peptides to induce Alzheimer's disease related symptoms in cells and mice. Some of the peptides are being acetylated on one end, which "may improve stability in in vitro and in vivo assays" according to Sigma, but no further explanations or reference were given.
I was wondering if this "improved stability" is specially designed for animal injection or cell culture treatments, or simply to make the peptide easier to handle.
What are the general purposes for acetylated peptides and should I use it in experiments, or the original peptide instead?
Thanks in advance. Lin
Dear Lin Zhou.
Amyloid-Beta 1-42 sequence favors towards hydrophilicity, so it get denature fast and unstable due to high moisture absorption nature. and always looking to fold itself. so for long storage purpose they are protecting highly active -NH2 group for it's stability.
Only for R&D non-human use purpose this type of modified peptide possible for use. If you are going to do your work for biological application for animal and human injection means you need to use free NH2 & COOH Terminal group.
And another thing. for some research need to do with only C-terminal, N-terminal need to off, so they want N- acetylated peptide.
So for your experiment based on condition you use unprotected original peptide. and i have lot of experience by synthesizing amyloid-Beta peptide and many other biological peptide and modifications.
If you need any peptide service and research help feel free to get it from us
Hope you may clear.,
Good Luck & Regards
R.Selva....
www.gmrfoundation.com
Protein N-terminal acetylation has multiple roles in many aspects of the protein life, such as assembly, stability, activity, and location. also in order to to remove electric charge, peptide ends are often modified by N-terminal acetylation. application may include intracellular, in-vivo assays, in-vivo or in-vitro functional studies. I think you can use them in your experiments.
Hi Lin, here is a list of advantages that peptide companies make about N- acetylation (and C-amidation)
http://www.greiner-bio-one.co.jp/products/peptides/acetylation_amidation.pdf
The only point that makes sense to me (for in vivo experiments) is that N-acetyl peptides are not cleaved by aminopeptidases.
Dear Lin Zhou.
Amyloid-Beta 1-42 sequence favors towards hydrophilicity, so it get denature fast and unstable due to high moisture absorption nature. and always looking to fold itself. so for long storage purpose they are protecting highly active -NH2 group for it's stability.
Only for R&D non-human use purpose this type of modified peptide possible for use. If you are going to do your work for biological application for animal and human injection means you need to use free NH2 & COOH Terminal group.
And another thing. for some research need to do with only C-terminal, N-terminal need to off, so they want N- acetylated peptide.
So for your experiment based on condition you use unprotected original peptide. and i have lot of experience by synthesizing amyloid-Beta peptide and many other biological peptide and modifications.
If you need any peptide service and research help feel free to get it from us
Hope you may clear.,
Good Luck & Regards
R.Selva....
www.gmrfoundation.com
I also agree with selva, acetylated peptide for stability improvement and storage purpose, and some reaction n terminal should need to off so they use that protected one.,
For you original sequence was recommended.
Almost Selva Chemist given clear answer for your question.
for application purpose you need to use original peptide.
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