Dear community,
I recently came across the NanoSuit® to prepare samples for scanning electron microscopy.
https://www.nature.com/articles/s41374-019-0309-7.pdf?origin=ppub
https://www.emsdiasum.com/microscopy/products/sem/nanosuit.aspx
In a nutshell, instead of the time-consuming conventional preparation steps (chemical fixiation and so) you simply coat your specimen with a few µL of the NanoSuit® aqueous solution, which is electron conductive. Additionally, it enables the researcher to investigate living organisms in SEM. Both are large advantages over traditional methods.
This almost sounds to good to be true. Therefore, we would like to give it a try. Unfortunately, the products price is really high (5 mL costs ~1000 $).
So here is the question: does anyone outside the lab of the inventor already has experience with the NanoSuit®? Is it really that good and powerful? Or
Best regards,
Uwe
Hello,
At the beginning of the NanoSuit ® story was the 1% tween20 in aqueous solution:
https://www.pnas.org/content/110/19/7631
https://academic.oup.com/jmicro/article/63/4/295/2804344
Article A ‘NanoSuit’ surface shield successfully protects organisms ...
Then came a mixture of sucrose, fructose, sodium chloride, citric acid, sodium glutamate and glycerine:
Article A modified ‘NanoSuit®’ preserves wet samples in high vacuum:...
You may first try homemade nanosuit.
I was following that few years ago but I didn't try it. At this time I took another direction for correlative microscopy, trying graphene oxide layer with a master student:
https://www.nature.com/articles/ncomms8384
Hi Nicolas,
thank you very much for your answer. As you suggested, we will now try homemade nanosuit. For sure, I will give a short report here on how it worked.
Cheers,
Uwe
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