I am designing a model of co-cultures and I would like to create epithelial-endothelium interactions. Has anyone experience with the cell line Hulec-5a? Is it a good model to evaluate endothelial dysfunction and make adhesion tests?
Vertical agriculture offers an innovative solution to reduce the pressure of cultivation on soils. The intensive use of machinery and large areas of land harm biodiversity.
28 October 2020 8,773 3 View
Hello, I am trying to find antibodies that can be used in paraffin rat brain sections against S100B , PSD95, Synapsin-I, VGLUT1,VGLUT2. I am checking the technical info provided by some companies...
12 August 2020 5,382 2 View
There are a lot of new ideas in different fields, but only few will be innovative (new+comercialiced). As an example, Escalators and moving walks have the same mechanical design from more than...
13 July 2020 7,807 12 View
Imagine SARS-COV-2 in a surface to be recovered with an hyssop .
08 July 2020 1,193 3 View
I'm thinking about a turn from my ethnographic approach to a phenomenological approach in the study of sustainability and forest preservation. However, I didn't find any good manual to design and...
06 July 2020 9,618 1 View
I think it would be interesting to implement the propagation of the COVID-19 virus in some simulation software for the circulation of people, for a population diverse in ages, heights, etc.,...
30 June 2020 7,481 14 View
It should be mandatory to learn from what life teaches us. With COVID-19, we have been forced to buy sanitary materials because in our country there was no industry to do so (dependency) and we...
30 June 2020 3,018 36 View
If you need a distribution map from a book, how do people deal with that? Do u email authors to obtain the layers or is there a way to export the polygons from the book images? Colleagues and I...
20 June 2020 4,391 1 View
I'm looking for information regarding the dinamic of lignin accumulation during in vitro culture of plants.
16 April 2020 3,699 5 View
For linear regressions between linear measurements and body mass, which transformation is better to use? base e logarithm (LN)? or base 10 logaritm (LOG10). Apparently they do not make such a for...
08 April 2020 5,201 12 View
I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...
03 March 2021 2,297 3 View
02 March 2021 3,060 3 View
Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...
02 March 2021 6,865 1 View
Is there a powerful system for the security of the systems distributed on IoT systems?
02 March 2021 3,858 10 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...
01 March 2021 2,272 4 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View