applicability - can be evaluated using different matrices ( your template, enzyme can be altered) with various concentrations. It should show similar results for as many matrices as possible.

practicability - blind samples can  be analysed by the routine assay method.  It can be further evaluated by transferring the method to a second laboratory where the experiment can be performed under reproducibility conditions.

Hi

maybe a quick read of this doc could be a good starting point

http://www.afsca.be/laboratories/labinfo/_documents/2013-07_labinfo10-p04_en.pdf

best regards

Thank you for your answers) I found a checklist which can be used for all stepsduring PCR validation - again, it is good for start.

But stil an example of PCR Validation Report for these parameters will be really very appreciated :)

Hi, thank for you image of checklist. I am attaching the paper of your checklist.

http://www.sciencedirect.com/science/article/pii/S0924224414000661

http://www.sciencedirect.com/science/article/pii/S0924224414000661

Hi,

Yes I do have experience in validations of Q PCR using specific target probes & i have experience in back cross validations of micro array datas on QPCR.I have standardised & optimised the gene specific probes for cancer detection (IL6 & TNF series) & for ABHD11 william syndrome detections.These probes are back validated & we are yet to publish in journals.

Across my different stints,I personally handled these QPCR platforms : Applied biosystems (Mx300p),Roche mini cycler,Bio Rad & Eppendorf real-time cyclers.

We as a team are also validating few gene series responsible for biofuels & renewable energy productions.Please contact us further to discuss & for any technical support on : [email protected].

Thanks,

Vivek