To what amount can you dilute matrigel? It to inject PDX subcutaneously into mouse. Undiluted it's almost impossible to work with, but overdilution leads to matrigel being incapable to solidify. What are the best practices?
Hello Pavel Nesmiyanov
The Matrigel may be diluted to the desired protein concentration using the lot-specific protein concentration provided on the certificate of analysis to determine the optimal protein concentration range for your application using ice-cold media.
You may mix the contents of the vial by pipetting up and down and the calculated amount of Matrigel matrix may be added to ice-cold media in pre-chilled glass tube held in ice. The diluted sample may be vortexed gently and held in ice. Generally, for in vivo applications, you should not dilute Matrigel to a final concentration below 4 mg/ml. Also, do not dilute by fold dilution, but instead try to dilute to a specific concentration (in mg/ml).
Best.
I inject subcutaneously PDX with undiluted matrigel and I dont have any problems. The matrigel is kept on ice and then mixed with the PDX. I dont use any syringe, I use a Trocar which is much larger and allow matrigel to get through.