Hello, did somebody check what could be the temperature of agarose in a tube when melted in boiling water bath?
Hi, Research gate! I need HELP! I'm trying to prepare nickel nanoparticles for my devices. I use the commonly used method from the article (DOI: 10.1002/adma.201405391). Briefly, 20 mmol...
31 March 2024 7,168 1 View
Has there been any research on managing talents who have excelled in competitions in mathematics, programming, or physics/chemistry/biology? How can we ensure that they make their most valuable...
28 February 2024 6,003 0 View
To what amount can you dilute matrigel? It to inject PDX subcutaneously into mouse. Undiluted it's almost impossible to work with, but overdilution leads to matrigel being incapable to solidify....
23 January 2024 6,509 2 View
Hello. The software which is used to simulate Brownian dynamics was already discussed here...
15 January 2024 7,904 0 View
Have anybody measured a kinetics of enzymatic digestion of BAEE(n-alpha-Benzoyl-Arginine Ethyl Ether) with USP method? I have observed acceptable delta-OD when use significant amounts of enzyme...
24 December 2023 9,366 1 View
I've been trying to isolate a low copy (CEN/ARS) plasmid from yeast that has selective markers for both yeast and bacteria (Trp and Amp). However, when I try to recover the plasmid I can't get any...
20 October 2023 6,749 2 View
I want to examine whether there is a significant difference between two data sets (the same number of participants, continuous variable). One has parametric distribution and another has...
06 September 2023 7,342 0 View
Do I understand correctly that heterozygous Pmel mice have twice as less cells that carry anti-gp100 TCR? This should be right because of the allelic exclusion. If so, can one combine both homo...
05 September 2023 259 1 View
I plan to present my findings on the conference. After that, I want to write a full-text article and submit it to the journal. About 90% of results will be new, while remaining 10% will be from my...
03 September 2023 4,108 3 View
We have an oldschool PPMS equipment with ACMS option, and recently got the problem with DC measurements. When starting a measurement it returns: Measuring DC Extraction 8/1/2023 10:54:37...
31 July 2023 4,244 1 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,684 1 View
is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it
06 August 2024 946 1 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,129 2 View
Do these peaks in the curve indicate primer dimer or non-specific products?
01 August 2024 7,465 0 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,672 4 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,121 4 View
I start with IDT ordered 124 bp ssDNA that is PCR amplified, then I use a QIAgen agarose gel extraction to get my exact product, and I'm trying to purify ~95bp RNA by denaturing PAGE to prevent...
16 July 2024 4,604 0 View
Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...
12 July 2024 2,058 5 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,667 5 View