Does anyone have experience freezing/thawing mouse lymph nodes?

10 September 2022 0 1K Report

We frequently freeze down mouse spleenocytes and thaw them for use in AIM and ICS but we have yet to do this for lymph nodes. I'm concerned that freezing low cell numbers and the fat surrounding the lymph nodes could effect cell viability when freezing/thawing. Does anyone have any good protocols for this they would be willing to share? Thank you!

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