PCR water or molecular grade water for RNA samples?

Hello everyone, It's my first time working with RNA but I am told it's very easily degraded. A protocol for cDNA synthesis requires "nuclease free water". Should I use PCR water or molecular grade...

27 May 2020 9,480 11 View

How to plot eye tracking data on video?

We collected eye tracking data as participants went through an hour's worth of gameplay for which we have video captures. How would I go about plotting the raw eye data on these videos so I can...

26 February 2020 1,585 2 View

Biosafety cabinet for RNA extractions necessary?

I am extracting RNA from milled plant material (BSL1) for downstream RNAseq. If possible, it would be great not to take over the only biosafety cabinet in the lab for RNase free space for weeks...

11 September 2019 8,712 4 View

Supervised classification in R?

I would like to perform supervised classifications in R on a large dataset of photographs to assess land cover change. Has anyone attempted this for landscape photographs in R? And is there value...

24 May 2018 4,522 4 View

We found a Cronbach's Alpha above 0.60 in 4 dimensions of our Questionnaire. The others 4were below 0.60. What should we do to avoid disrupting the Q?

We are building an almost "standardized" questionnaire according to Ellen G. White on the 8 natural remedies. When doing the factorial analysis, we found 4 dimensions with Alpha of Cronbach above...

17 May 2018 8,024 5 View

No title

I need some articles that can help me understand electromagnetism in different animals.

04 September 2016 2,921 3 View

What is a Likelihood ratio test with 0 degree of freedom?

Would the results from a likelihood ratio test with 0 degree of freedom not be interpretable? From what I understand, by definition when the degrees of freedom = 0, chi-squared = 0 thus making the...

22 November 2015 3,767 12 View

Where to find a dietary recall?

Looking for a 24 hour dietary recall.

25 March 2014 6,130 3 View

Can you define 21st century learners?

I think millenials (because I'm old and my kids are millenials) but also because I think the teaching methods that have been used in the US, the learning standards, are so outdated, boring, and I...

01 January 1970 4,544 12 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

I want to simulate a system containing protein and heavy metals; which force field is most reliable for this purpose?

The protein I want to simulate has multiple heavy metals. I wanted to know which force-field I should use with GROMACS to achieve this. Most importantly, do the latest updates in the force-field...

16 February 2021 2,420 1 View

Passivation of chromium cast Iron in high temperature?

14 February 2021 2,157 3 View

What can be use to mop up heavy metals from vegetables or soils in quarry mining site?

It has been established that quarry mining site polluted and contaminated it's environment with heavy metals, these affects the crops and vegetables planted in the area. The possible solutions to...

12 February 2021 1,155 5 View

Please how can i write a proposal on the methodolgies of AAS to determine heavy metalsin water?

Please I want to write a proposal on the methodologies of AAS to determine heavy metals in water. please any link can help.

08 February 2021 3,587 7 View

How to calculate the Boiling point for metals at high vapor pressure?

In our experiment, some metal surfaces are exposed to radiation heat under high vapor gas pressure. I would like to ask if there is any way to calculate the boiling point for metals under high...

31 January 2021 249 4 View

How can I prepare 300mg/L concentration of lead(II) nitrate ( Mol. Wt reffered is 331.21) incoorporated Luria-Bertani (LB) agar ?

I need to check the Heavy metal (lead) consumption assay using microbes at varying concentration in same media. ( 200mg/L to 2000mg/L).kindly help me to clear it.

19 January 2021 1,946 9 View