PCR water or molecular grade water for RNA samples?

Hello everyone, It's my first time working with RNA but I am told it's very easily degraded. A protocol for cDNA synthesis requires "nuclease free water". Should I use PCR water or molecular grade...

27 May 2020 9,480 11 View

Biosafety cabinet for RNA extractions necessary?

I am extracting RNA from milled plant material (BSL1) for downstream RNAseq. If possible, it would be great not to take over the only biosafety cabinet in the lab for RNase free space for weeks...

11 September 2019 8,712 4 View

Supervised classification in R?

I would like to perform supervised classifications in R on a large dataset of photographs to assess land cover change. Has anyone attempted this for landscape photographs in R? And is there value...

24 May 2018 4,522 4 View

We found a Cronbach's Alpha above 0.60 in 4 dimensions of our Questionnaire. The others 4were below 0.60. What should we do to avoid disrupting the Q?

We are building an almost "standardized" questionnaire according to Ellen G. White on the 8 natural remedies. When doing the factorial analysis, we found 4 dimensions with Alpha of Cronbach above...

17 May 2018 8,024 5 View

No title

I need some articles that can help me understand electromagnetism in different animals.

04 September 2016 2,921 3 View

What is a Likelihood ratio test with 0 degree of freedom?

Would the results from a likelihood ratio test with 0 degree of freedom not be interpretable? From what I understand, by definition when the degrees of freedom = 0, chi-squared = 0 thus making the...

22 November 2015 3,767 12 View

Where to find a dietary recall?

Looking for a 24 hour dietary recall.

25 March 2014 6,130 3 View

Can you define 21st century learners?

I think millenials (because I'm old and my kids are millenials) but also because I think the teaching methods that have been used in the US, the learning standards, are so outdated, boring, and I...

01 January 1970 4,544 12 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

No module named 'tensorflow.keras.backend.tensorflow_backend' ?

System Description Cuda 11.2 GeForce GTX 1080ti tensorflow version 2.4.1 keras version 2.2.0 python version 3.8 I was getting the below error when I try to run my code on the...

25 February 2021 6,673 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View