I have two sites that have different claims in their ability to have succesful cDNA libraries from RNA with RIN scores:
1) UC Davis (https://dnatech.genomecenter.ucdavis.edu/tag-seq-gene-expression-profiling/) wants a a RIN score of 6 or higher.
2) A kit from GenX Pro (https://genxpro.net/sequencing/transcriptome/mace-massive-analysis-of-cdna-ends/) that says a RIN of 3 or higher is doable.
For those that have either used this MACE kit, followed protocols similar to Lohman et al (Article Evaluation of TagSeq, a reliable low-cost alternative for RNAseq
), Meyer et al (Article Profiling gene expression responses of coral larvae (Acropor...
), or the modified Meyer on the Matz Lab website.... how low of a RIN have you successfully created a cDNA library for sequencing?
Gina Chaput RIN score is very important. If you will compromise with RIN score that means you will lose capturing of many genes. In practice 6+ is OK but based on my experience I would suggest 7.5+ in case of human.
Always try to have the highest RIN possible - it is crucial for your sequencing. With a low RIN, you will miss a ton of genes, and the sequencing will not be clean. You will also always lose RIN during the time you prepare for sequencing or transport the samples. I would recommend sequencing only with RIN higher than 7, but 8-9 would be much better. Do you have some trouble with obtaining a high RIN score? What is the problem? Are the samples quite degraded, or is there something wrong with the isolation?
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