Does anyone have experience about percentage of FITC labelled bacteria?

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Vishnu Rompicharla

How are you calculating the percentage of FITC labelled as 30% ? I assume you have added FITC to bacterial suspension, incubated, centrifuged and removed the supernatant to check unlabelled FITC. If so, that may not be the correct way of quantifying percentage of bacteria stained. Share your protocol in brief?

Abdullah Al Tarique

Vishnu Rompicharla I am facing the same problem of labelling live bacteria. Could you please share, how to label live bacteria?