6 Questions 20 Answers 0 Followers
Questions related from Abdullah Al Tarique
I digest my plasmid with KpnI-NotI. I cut the expected 4kb band from the gel, purified it and then I did ligation [1:3 and 1:5 vector:insert ratio] and transformation. I found 18 transformed...
01 July 2024 3,992 0 View
My vector size is 5.1kb and insert size is 4kb. Initially, I performed digestion of the insert from a different plasmid and the vector using KpnI-NotI. I checked them on the gel, excise and...
20 May 2024 3,205 8 View
My vector size 5.1kb and insert size is 4kb. I performed double digestion of vector and insert, ran them on the gel and then extracted them from the gel. 260/280 ratio was good. Then I did...
07 May 2024 8,326 11 View
While preparing E. coli DH5alpha competent cells, most of the methods describe using E. coli DH5a at OD 0.4-0.5. Is there any specific reason for using OD 0.4-0.5? If OD is more than 1, will it be...
18 April 2024 2,314 3 View
I am looking for antibody against human cell membrane which will circularly stain the cell membrane only. Could anyone name such antibody? I want to avoid F-actin, microtubulin bcz they stain all...
23 January 2018 5,231 11 View
Is it possible to separate human M1 and M2 macrophages by flow cytometry? Has anyone done this? Could anyone provide any related literature?
10 November 2013 4,980 14 View
