During DNA gel purification using a centrifugation technique, I accidentally centrifuged the sample at 1300 rpm instead of 13,000 rpm. What will be the consequence? The gel along with DNA weighed 0.292 gms and I did the same mistake in all steps.
Have you checked your product (the output of the purification) on a gel again? Have you lost it or is it still there? If you are using spin columns and still have them you might be lucky that the DNA is still sticking to the membrane (some people keep the columns, the material sticking to the column is enough for PCR/cloning in many cases if your product is lost). In that case just repeat the elution steps with the correct speed, DNA is quite tough and survives on the column.
If you did a precipitation assay without spin column and discarded the supernatant the DNA is probably lost.
Good luck!
If you have used commercial column, you can add a small volume of elution buffer and spin again. Henceforth, don't throws supernatants especially when it is a valuable sample.
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