I have been trying to establish a standard curve for ethanol by HPLC. I have tried water:acetonitrile:methanol as mobile phase and obtained various peaks and I am not able to identify the peak for ethanol. The column that I have is a C18 column. I need to know the composition of the the mobile phase to determine ethanol by HPLC and expected retention time of ethanol. Thanks in advance.
Hello,
You should try GC analysis instead of HPLC.
In fact, EtOH can be used as a mobile phase in HPLC. You cannot retain this molecule on a C18...
Best regards,
Laurent
Parvathi Balasubramanian, your proposed method does not make sense. *HPLC is not an appropriate technique to measure ethanol in solution with. Gas chromatography (or even Refractive Index via refractometer !) should be explored once the sample type and goals have been described.
The proper analytical technique(s) should be initially identified and used to answer the question posed. First, research your question so you understand exactly what it is you wish to determine.
Next, specify:
- how much ethanol is expected to appear in your sample type AND also what other chemicals may be present (IOW: What is the ethanol mix in with?).
This is critical to understand as different techniques may be applicable depending on the type of sample you wish to analyze.
Laurent Collard Thank you for the response. Will proceed with GC analysis.
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