Abheepsa Mishra

4 Questions 9 Answers 0 Followers

Questions related from Abheepsa Mishra

How to plot a graph representating Topological Associating Domains (TADs), Normalized tag counts, and log2 FC (RNA Seq data) in GraphPad Prism?

Is there any other software to plot TADs ( I have chromosome start and end position), normalized tag count from ChIP-Seq data, and log2FC of genes (fold change from RNA seq data) in one graph?

08 August 2018 1,885 1 View

Presently, I have synthesized a cyclic octapeptide, how can I predict/or optimize its structure using bioinformatics tools?

I have used CycloPs and ACD/chemsketch to generate virtual structure of peptide (pdb format). Is it necessary to optimize the structure or do energy minimization studies before proceeding to...

11 November 2013 6,087 5 View

How to sequence cyclic peptides by MALDI-ToF-PSD or LC-ESI-MS?

My peptide is not getting fragmented by ESI-MS. The peptide mass is below 1kDa. I have already checked for Cys residue. I have not pretreated my sample with enzyme or 6 N HCl. Is there any...

09 September 2012 1,770 4 View

Tricine SDS-PAGE

My separating gel is not getting polymerized. (It does not set). I am following protocol by Schagger, Von Jagow protocol. Can anyone please forward me your protocol for Tricine SDS PAGE?

08 August 2012 8,058 7 View