What is a common method for modelling evanescent wave-related phenomena, especially in dispersive media?

07 August 2014 7,968 3 View

How can I measure pH in microvolume without a pH meter?

04 May 2014 3,389 4 View

Why is my Thrombin and TCEP mixture resulting in turbidity and gelling?

After mixing Thrombin (2.5mM) with TCEP (30mM) in PBS buffer and waiting for an hour at room temperature I observed a significant turbidity and a slight gelling of the solution. Thrombin was...

01 February 2014 3,362 5 View

Could protein disulfide bond conformations differ in X-ray structure (monocrystalline) and lyophilized powder?

I was measuring lyophylized bovine thrombin (purchased form Sigma Aldrich, http://www.sigmaaldrich.com/catalog/product/sigma/t4648 : composition

09 October 2013 1,534 4 View

Oxidized DTT RAMAN activity?

A useful tool for disulfide bridges reduction, for example in proteins, is DTT. As a product of these reductions (in attachment) we have an "oxidized DTT"...

03 April 2013 8,328 3 View

Evidence of disulfide bridges frequencies in Raman spectra of proteins below 500cm-1

There is theoretical possibility of disulfide bridges confirmation with Raman frequencies from 485cm-1 to 500cm-1, but it is a bit unusual. There are even experimental proofs for some model...

02 March 2013 3,870 4 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

How to troubleshoot Electrochromic Films and Electrochromic Devices?

I am trying to characterize an electrochromic system. The system uses ITO electrodes on glass, WO3 as the electrochromic film, and NiO as the ion storage layer. When I deposit NiO and WO3 and...

23 February 2021 9,592 2 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

No title

22 February 2021 9,932 11 View