Vasily N Kruzhilin

7 Questions 11 Answers 0 Followers

Questions related from Vasily N Kruzhilin

Does anyone have any experience with the Ultra-M micro pH electrode?

Hi! Does anyone have any experience with the Ultra-M micro pH electrode by 120Scientific, a division of Lazar Research Laboratories, Inc. (http://bio.120scientific.net/)? Could anyone give a short...

08 August 2014 2,736 0 View

What is a common method for modelling evanescent wave-related phenomena, especially in dispersive media?

Hi! Can I use FDTD method or there are some limitations?

08 August 2014 7,995 3 View

How can I measure pH in microvolume without a pH meter?

Please could you give me an idea of how to measure pH in a volume ~100 µl if I don't have an appropriate pH meter? I had an idea to use fluorescent ph-sensitive tags.

05 May 2014 3,427 4 View

Why is my Thrombin and TCEP mixture resulting in turbidity and gelling?

After mixing Thrombin (2.5mM) with TCEP (30mM) in PBS buffer and waiting for an hour at room temperature I observed a significant turbidity and a slight gelling of the solution. Thrombin was...

02 February 2014 3,405 5 View

Could protein disulfide bond conformations differ in X-ray structure (monocrystalline) and lyophilized powder?

I was measuring lyophylized bovine thrombin (purchased form Sigma Aldrich, http://www.sigmaaldrich.com/catalog/product/sigma/t4648 : composition

10 October 2013 1,561 4 View

Oxidized DTT RAMAN activity?

A useful tool for disulfide bridges reduction, for example in proteins, is DTT. As a product of these reductions (in attachment) we have an "oxidized DTT"...

04 April 2013 8,372 3 View

Evidence of disulfide bridges frequencies in Raman spectra of proteins below 500cm-1

There is theoretical possibility of disulfide bridges confirmation with Raman frequencies from 485cm-1 to 500cm-1, but it is a bit unusual. There are even experimental proofs for some model...

03 March 2013 3,928 4 View