Does anyone have any experience with the Ultra-M micro pH electrode?

07 August 2014 2,705 0 View

How can I measure pH in microvolume without a pH meter?

04 May 2014 3,389 4 View

Why is my Thrombin and TCEP mixture resulting in turbidity and gelling?

After mixing Thrombin (2.5mM) with TCEP (30mM) in PBS buffer and waiting for an hour at room temperature I observed a significant turbidity and a slight gelling of the solution. Thrombin was...

01 February 2014 3,362 5 View

Could protein disulfide bond conformations differ in X-ray structure (monocrystalline) and lyophilized powder?

I was measuring lyophylized bovine thrombin (purchased form Sigma Aldrich, http://www.sigmaaldrich.com/catalog/product/sigma/t4648 : composition

09 October 2013 1,534 4 View

Oxidized DTT RAMAN activity?

A useful tool for disulfide bridges reduction, for example in proteins, is DTT. As a product of these reductions (in attachment) we have an "oxidized DTT"...

03 April 2013 8,328 3 View

Evidence of disulfide bridges frequencies in Raman spectra of proteins below 500cm-1

There is theoretical possibility of disulfide bridges confirmation with Raman frequencies from 485cm-1 to 500cm-1, but it is a bit unusual. There are even experimental proofs for some model...

02 March 2013 3,870 4 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Why the results when using wall thickness and when using shell conduction are different in ansys fluent?

Actually, I am running cfd simulation for a heat exchanger which has two fluids one hot and other one is cold fluid. there is a solid domain between them, which I removed and instead I have used...

01 March 2021 9,537 2 View