Protein Quantification Outside Standard Curve?

I am running a Bradford protein assay and my protein values are falling beyond the standard curve. I am already using 1uL of sample in the standard microplate assay. Is there a way to extend the...

13 December 2020 6,672 3 View

How to analyze sodium lauryl sulfate using HPLC-RID?

Hi, I am analyzing the content of anionic surfactant: sodium lauryl sulfate (SLS) in commercial detergents using HPLC-RID/refractive index detector. I used column Agilent Zorbax Eclipse XDB C-18...

15 November 2020 1,405 2 View

Cs-Gluconate for intracellular solution?

Dear patch-clamp enthusiasts, For patch-clamp recordings of vomeronasal sensory neurons, I need a cesium-based intracellular solution. Because I also need a defined amount of chloride, I have to...

11 November 2020 6,384 7 View

PLS- Is it normal that the standardized regression coefficients of my model do not coincide with the VIP values?

I am trying to perform a partial least square model (PLS) using one response variable and 33 explanatory.. Before the analysis I have standardized all my data so i am expecting that the regression...

19 October 2020 6,702 2 View

Which is preferable for whole rat brain clearing: tert-butanol or xylene?

I am performing LM on whole rat brains with a specific focus on the hippocampus and cannot find which information on which clearing technique is better.

05 October 2020 1,306 1 View

Is recoding negative questions always necessary when downloading to SPSS from SurveyMonkey?

I formatted a survey in SurveyMonkey using questions from a valid and reliable instrument (Principles of Adult Learning, Conti, 1978). Questions used a 6 point Likert scale (Always, Almost...

29 September 2020 8,194 4 View

Clarification on Shapiro-wilk sig. , skewness and kurtosis?

Hello, Maybe is a bit silly question but, I have a variable (N=78) and on shapiro-wilk test the sig. is .008 which is just over (greater) than 0.05 meaning that is just about normally...

29 August 2020 1,384 4 View

Has anyone been able to quantify iodide using an AS28 column on an Integrion IC with hydroxide eluent?

20 August 2020 2,600 1 View

How to use Clamping and interpret its outcomes in MAXENT?

For my Masters project I have been creating SDMs using MAXENT, and the next stage involves using future climate projections in the 'projection layers directory' to see potential future...

25 June 2020 2,658 6 View

Google Earth Engine for Multitemporal analysis (Land Use Land Cover Change)?

Hello. I am reading about Google Earth Engine (GEE) and their applications for vegetation, landcover and agriculture mapping. I am analyzing an area of 20Mha to determine a multitemporal changes,...

01 May 2020 2,059 11 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View