Hi!
I´m currently trying to isolate Mitochondria from HCM cells. For my western blots I need an additional antibody against a mitochondrial protein which is bigger or smaller than 14/17 kDa.
I was thinking of a protein which is only present in mitochondria or in the matrix. Maybe transmembrane proteins (TOM or TIM) will work too, but I don't know if the membranes are still intact in my samples. Maybe proteins from the ß-oxidation? Does someone know which antibodies can be used to detect mitochondrial proteins in western blots? If possible only proteins/Abs that are located/synthesized in the mitochondria (matrix). Thanks a lot!
Hello Victoria Momand
You may use cytochrome c as a marker representing the mitochondrial intermembrane protein with molecular weight of 12kDa.
If you are interested in proteins located/synthesized in the mitochondria matrix, then you may look at hsp60.
The article attached below will be helpful.
Article Protocol for mitochondrial isolation and sub-cellular locali...
Best.
To detect mitochondria in Western blots, you typically use antibodies targeting proteins specific to mitochondria. Here are some commonly used antibodies for detecting mitochondria:
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