Victoria J Forster

6 Questions 12 Answers 0 Followers

Questions related from Victoria J Forster

Doing RNAseq and genome-wide methylation analysis on a small number of cells - any suggestions?

Hi all,Looking for some advice. I have a small number of iPSC-derived oligodendrocytes (approx 10,000 cells/sample) that I'd like to do RNAseq and whole genome wide methylation analysis (e.g....

17 April 2017 1,984 1 View

Functional myelination assays with oligodendrocytes/axonal neuron cell lines?

Hi all, I'm doing a project on drug-induced neurotoxicity but my background is in leukaemia biology, so I'm very much learning as I go along with regards to neuroscience, neural cell lines etc....

26 March 2016 5,800 3 View

Trying to optimise the PIG-A flow cytometry based mutation assay in the myeloid cell line HL-60, can anyone lend their expertise?

I am trying to modify a mutation assay I have had success with in other suspension cell lines - PIG-A and have encountered some difficulties that I am hoping to troubleshoot with you all. PIG-A...

28 May 2014 7,227 1 View

Does anyone have any experience of HUVEC proliferation/migration/tube formation assays?

Am hoping to use HUVEC for the first time in co-culture assays. I have never used them before, so I'm just wondering if anyone has any recommended assays to investigate any of the above? Also,...

15 May 2013 3,137 8 View

I am using competent E.coli which are EndA+, how do I prevent DNA shearing when preparing my plasmid DNA?

I am trying to create a 16kb construct and transform Stbl3 competent E.coli with the construct. However during restriction digest analysis of my possible constructs, I either see banding patterns...

02 April 2013 3,650 13 View

Does anyone have any experience of co-culturing HUVECs (or similar) and primary immortalised haematopoietic stem cells?

Investigating whether it would be possible to do this at least for a few days/weeks. Would appreciate it if anyone has any experiences they would be willing to share. Or even hints and tips for...

20 March 2013 1,658 1 View