Victoria J Forster

4 Questions 8 Answers 0 Followers

Questions related from Victoria J Forster

Doing RNAseq and genome-wide methylation analysis on a small number of cells - any suggestions?

Hi all,Looking for some advice. I have a small number of iPSC-derived oligodendrocytes (approx 10,000 cells/sample) that I'd like to do RNAseq and whole genome wide methylation analysis (e.g....

17 April 2017 1,954 1 View

Functional myelination assays with oligodendrocytes/axonal neuron cell lines?

Hi all, I'm doing a project on drug-induced neurotoxicity but my background is in leukaemia biology, so I'm very much learning as I go along with regards to neuroscience, neural cell lines etc....

26 March 2016 5,773 3 View

I am using competent E.coli which are EndA+, how do I prevent DNA shearing when preparing my plasmid DNA?

I am trying to create a 16kb construct and transform Stbl3 competent E.coli with the construct. However during restriction digest analysis of my possible constructs, I either see banding patterns...

02 April 2013 3,600 13 View

Does anyone have any experience of co-culturing HUVECs (or similar) and primary immortalised haematopoietic stem cells?

Investigating whether it would be possible to do this at least for a few days/weeks. Would appreciate it if anyone has any experiences they would be willing to share. Or even hints and tips for...

20 March 2013 1,612 1 View