Does anyone have any experience in reducing use of animal products in the lab e.g. serum-free tissue culture and trypsin alternatives?

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Tatsiana Rakovich

I don't have experience with those exact cells, but I used to use the SF Ultraculture medium to grow HepG2 cells and instead of Trypsin we used TrypLE and Accutase. Hope that helps.

Harriet C T Groom

thanks Tatsiana!

Alan Coulson

We developed a serum free medium to culture human lymphocytes. The papers are on my site. They might be helpful to you, although they are very old!

Thank you. I will take a look. Harriet