23 February 2018 4 5K Report

16S rRNA profiling

More Harriet Ugboko's questions See All
How do I purify serum in unconventional way?

I plan to purify serum for serum-neutralization assay, by simply centrifuging the serum, collecting the supernatant, and discarding the debris. Has anyone ever done this before? If so, what speed...

05 February 2023 3,853 3 View

What is the best protocol for isolating neutrophils for investigating Mac-1 surface expression?

Hi, I have been isolating neutrophils for N-Formylmethionyl-leucyl-phenylalanine (FMLP) activation for a couple of months without success. In my protocol, I dilute heparinized whole blood with PBS...

15 May 2022 2,964 0 View

Is there any pilot study done on the validity of hamzat and dada questionnaire?

I'm looking for the validity and reliability of the questionnaire used in this study ' wheelchair accessibility to public buildings by people living with disabilitys' by hamzat and dada

25 February 2022 3,397 0 View

How does Programmatic Advertising relate to Geodemographics?

They are both within the sphere of contemporary digital marketing, each a different technique I guess, I'm just wondering what the direct links between them are? Do any programmatic advertising...

08 November 2021 9,220 1 View

Would you grant me permission to use your images of cubozoans?

I am working on a guide to invertebrates in the northern Gulf of Mexico for use on federal and state survey vessels to help improve identifications at sea. I am asking for permissions to use one...

01 September 2020 7,137 1 View

Is there a rule about the frequency conversion in photon, phonon, magnon conversions in crystal lattices?

We are trying to understand the role of basalt paramagnetism in soil to enhance plant growth. Apparently ELF waves interact with basalt in a way that is favorable for plant growth. The question is...

19 July 2020 9,108 0 View

Can i get help in understanding and interpretation of multiple alignment of a sequence after cloning & blasting?

I am working on genome & chromosomal properties of Bambara groundnut. My interest is in centromeric DNA -tandem repeats. I used primers from cowpea which is a closely related specie to bambara....

20 August 2019 7,454 3 View

What might cause the ~160bp peak in this library prepared using FFPE extracted RNA and Takara SMARTer kit?

I have prepared libraries for RNA-seq using the Takara SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian. The input RNA was extracted from FFPE tissue. Some of the final libraries have...

16 June 2019 1,846 2 View

What type of statistical tool can I use for my research project?

My PICOT is as follows: for nurses at a jail setting, how does implementing a policy of EBP guidelines on TDM influence serum drug lab testing? i plan on comparing the percentage of completed...

15 March 2017 1,894 3 View

Does anyone have any experience in reducing use of animal products in the lab e.g. serum-free tissue culture and trypsin alternatives?

I am trying to eliminate or reduce animal product use in my lab, which mostly means tissue culture, as molecular biology products are mostly fine. Does anyone have any experience with serum-free...

09 August 2016 3,331 4 View

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

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Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

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I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

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