I plan to purify serum for serum-neutralization assay, by simply centrifuging the serum, collecting the supernatant, and discarding the debris. Has anyone ever done this before? If so, what speed and time did you spin at?
As far as I know, this is the standard method for preparing serum. You collect the blood in a tube without anticoagulants, leave it to clot, centrifuge out the clot and pipette off the serum. 5000 g for 10 minutes is standard, if I remember correctly from my days in the clinical chemistry lab. Any textbook of laboratory medicine should be able to provide the details.
I recommend 2000xg for 10 minutes. Look for thrombocytes in the debris.
from thermo fisher site
After collection of the whole blood, allow the blood to clot by leaving it undisturbed at room temperature. This usually takes 15–30 minutes. Remove the clot by centrifuging at 1,000–2,000 x g for 10 minutes in a refrigerated centrifuge. The resulting supernatant is designated serum. Following centrifugation, it is important to immediately transfer the liquid component (serum) into a clean polypropylene tube using a Pasteur pipette. The samples should be maintained at 2–8°C while handling. If the serum is not analyzed immediately, the serum should be apportioned into 0.5 ml aliquots, stored, and transported at –20°C or lower. It is important to avoid freeze-thaw cycles because this is detrimental to many serum components. Samples which are hemolyzed, icteric or lipemic can invalidate certain tests.
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