Does anyone have any recommendation for microscopy-based assay for T-cell lytic function?

We are working with primary human T cells and are searching for a good assay to look at their lytic function. The chromium release assay is an option, but we're looking to avoid radioactivity...

07 August 2016 407 1 View

Does anyone have any advice for running primary human monocytes on a western blot?

19 July 2016 1,036 5 View

Does anyone have any advice for running primary human monocytes on a western blot?

06 July 2016 9,137 6 View

How is the chamber removed in BD Biocoat 8-well cell culture slides?

Has anyone used BD Biocoat 8-well cell culture chambers before? If so do you have any advice on how to remove the chamber without soaking in an alcohol? I cannot permeabilize the cells I am...

10 November 2014 7,816 5 View

How is the chamber removed in BD Biocoat 8-well cell culture slides?

Has anyone used BD Biocoat 8-well cell culture chambers before? If so do you have any advice on how to remove the chamber without soaking in an alcohol? I cannot permeabilize the cells I am...

03 November 2014 5,807 4 View

What is a good protocol to dry cells that have been seeded on slides for immunofluorescence?

I have come across a few protocols using different increments of ethanol, but most of these refer to tissue sections. Most others in our lab have also done this with tissue sections. I am using a...

11 December 2013 521 5 View

Has anyone noticed abnormal cell morphology when seeding on poly-D-lysine?

This is very likely a cell type-dependent phenomenon, but is this a common occurrence for cells to appear a bit different when grown on poly-D-lysine as opposed to other surfaces?

10 November 2013 1,820 5 View

Plasma membrane proteins and western blot

Is anyone familiar with plasma membrane protein aggregation in samples for western blot? I am getting good signal but the pattern makes no sense! I did some brief reading and it seems plasma...

26 October 2013 7,105 19 View

Plasma membrane proteins and western blot

Is anyone familiar with plasma membrane protein aggregation in samples for western blot? I am getting good signal but the pattern makes no sense! I did some brief reading and it seems plasma...

09 October 2013 6,587 13 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View