We have 2 conditions where the morphology and signal of several mitochondrial probes are different. I keep coming across caveats associated with both techniques when it comes to quantification. Any suggestions?
We are working with primary human T cells and are searching for a good assay to look at their lytic function. The chromium release assay is an option, but we're looking to avoid radioactivity...
07 August 2016 470 1 View
With primary monocytes/macrophages, we are getting a dirty signal with multiple bands and background using otherwise specific antibodies that usually give us one band. We recognize that flow...
19 July 2016 1,080 5 View
06 July 2016 9,188 6 View
Has anyone used BD Biocoat 8-well cell culture chambers before? If so do you have any advice on how to remove the chamber without soaking in an alcohol? I cannot permeabilize the cells I am...
10 November 2014 7,864 5 View
03 November 2014 5,852 4 View
I have come across a few protocols using different increments of ethanol, but most of these refer to tissue sections. Most others in our lab have also done this with tissue sections. I am using a...
11 December 2013 574 5 View
This is very likely a cell type-dependent phenomenon, but is this a common occurrence for cells to appear a bit different when grown on poly-D-lysine as opposed to other surfaces?
10 November 2013 1,867 5 View
Is anyone familiar with plasma membrane protein aggregation in samples for western blot? I am getting good signal but the pattern makes no sense! I did some brief reading and it seems plasma...
26 October 2013 7,144 19 View
09 October 2013 6,626 13 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View
Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...
31 July 2024 3,913 6 View
i am working on ischemic stroke. for immunoflourescence i want to use zeiss confocal microscope.before i used VS120 but the images from vs120 not so good now i want to use zeiss confocal but i...
30 July 2024 2,349 1 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View