Does anyone have any advice for running primary human monocytes on a western blot?

19 July 2016 1,036 5 View

Does anyone have any advice for running primary human monocytes on a western blot?

06 July 2016 9,137 6 View

How is the chamber removed in BD Biocoat 8-well cell culture slides?

Has anyone used BD Biocoat 8-well cell culture chambers before? If so do you have any advice on how to remove the chamber without soaking in an alcohol? I cannot permeabilize the cells I am...

10 November 2014 7,816 5 View

How is the chamber removed in BD Biocoat 8-well cell culture slides?

Has anyone used BD Biocoat 8-well cell culture chambers before? If so do you have any advice on how to remove the chamber without soaking in an alcohol? I cannot permeabilize the cells I am...

03 November 2014 5,807 4 View

Does anyone have any advice on quantification of mitochondrial probes using FACS vs. microscopy?

We have 2 conditions where the morphology and signal of several mitochondrial probes are different. I keep coming across caveats associated with both techniques when it comes to quantification....

03 April 2014 4,421 0 View

What is a good protocol to dry cells that have been seeded on slides for immunofluorescence?

I have come across a few protocols using different increments of ethanol, but most of these refer to tissue sections. Most others in our lab have also done this with tissue sections. I am using a...

11 December 2013 521 5 View

Has anyone noticed abnormal cell morphology when seeding on poly-D-lysine?

This is very likely a cell type-dependent phenomenon, but is this a common occurrence for cells to appear a bit different when grown on poly-D-lysine as opposed to other surfaces?

10 November 2013 1,820 5 View

Plasma membrane proteins and western blot

Is anyone familiar with plasma membrane protein aggregation in samples for western blot? I am getting good signal but the pattern makes no sense! I did some brief reading and it seems plasma...

26 October 2013 7,105 19 View

Plasma membrane proteins and western blot

Is anyone familiar with plasma membrane protein aggregation in samples for western blot? I am getting good signal but the pattern makes no sense! I did some brief reading and it seems plasma...

09 October 2013 6,587 13 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

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03 March 2021 5,360 2 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View