10 Questions 14 Answers 0 Followers
Questions related from A.M. V
We are working with primary human T cells and are searching for a good assay to look at their lytic function. The chromium release assay is an option, but we're looking to avoid radioactivity...
08 August 2016 453 1 View
With primary monocytes/macrophages, we are getting a dirty signal with multiple bands and background using otherwise specific antibodies that usually give us one band. We recognize that flow...
20 July 2016 1,064 5 View
07 July 2016 9,169 6 View
Has anyone used BD Biocoat 8-well cell culture chambers before? If so do you have any advice on how to remove the chamber without soaking in an alcohol? I cannot permeabilize the cells I am...
11 November 2014 7,852 5 View
04 November 2014 5,839 4 View
We have 2 conditions where the morphology and signal of several mitochondrial probes are different. I keep coming across caveats associated with both techniques when it comes to quantification....
04 April 2014 4,451 0 View
I have come across a few protocols using different increments of ethanol, but most of these refer to tissue sections. Most others in our lab have also done this with tissue sections. I am using a...
12 December 2013 550 5 View
This is very likely a cell type-dependent phenomenon, but is this a common occurrence for cells to appear a bit different when grown on poly-D-lysine as opposed to other surfaces?
11 November 2013 1,848 5 View
Is anyone familiar with plasma membrane protein aggregation in samples for western blot? I am getting good signal but the pattern makes no sense! I did some brief reading and it seems plasma...
27 October 2013 7,130 19 View
10 October 2013 6,609 13 View
