Does anyone have an SOP for dsRNA production in vitro or know where I could find one?

More Jamie Bojko's questions See All

18S PCR primers for Crustacea - do any exist?

Hey everyone, I was wondering if anyone has tested/published some crustacean focussed 18S primers that provide an amplicon size of around 1kb to use for phylogenetics? Many thanks!

08 September 2018 9,086 3 View

Has anyone managed to install BioLinux onto an external hard drive to use alongside Windows 10? Do you know of a help page?

I need to download BioLinux but down want to wipe my laptop. I have been told I can download onto a USB or external hard drive but I cant find a definitive method that walks me through the...

10 November 2016 4,902 0 View

Bt toxins that target and induce mortality in crustaceans?

Hi, I am currently looking into the development of Bt toxins for crustacean control and have come across zero articles that attempt to develop Bt toxins that target, and induce mortality in,...

03 April 2016 9,008 4 View

Does anyone know how I can acquire some of these amazing amphipods?

Hi there, I am interested in doing some parasitology study on these amazing, giant amphipods. Its a bit of a longshot but if anyone out there is able to get hold of some I would be very interested...

04 May 2015 9,825 3 View

Is anyone working on control methods of vertebrate pests in agriculture? - What methods are allowed to be used for control?

Thank you for looking at this question. I am interested to know what regulations are involved in creating a control plan for vertebrate pests and what differences are in place between these and...

08 September 2013 9,229 4 View

Many invertebrates are agricultural pests but which species are the most studied?

I am interested in control methods used to prevent pest populations causing havok to agricultural industry. In particular I am most interested in biological control methods. If anyone knows any...

04 May 2013 2,652 9 View

The Volga River in Russia: geography, invasive profile and pathogen presence. Is anybody able to provide information?

Future work has directed an interest to The Volga Rover in Russia. Is anyone able to provide me with information on the region and in particular the best time to sample macroinvertebrates? As...

03 April 2013 6,705 6 View

Have any parasitology studies been performed on Gecarcoidea natalis, the red land crab native to Christmas Island?

I hope to look into the parasitology of this species but so far have only come across a parasitic Drosophilid which has been noticed to lay eggs around the secondary antennae of this crab. Are any...

02 March 2013 9,714 2 View

Can anyone recommend a reliable Genome annotation software?

Pending work on annotating a viral genome (~1Mb) and a microsporidian genome (~7.5Mb) I hope to find a reliable genome annotation software which is capable of detecting a variety of genetic...

02 March 2013 9,797 11 View

Genomic Study Costs

Is anyone able to give me a ballpark estimate on the price required to sequence a DNA virus between 0.5-1.0Mb? The same question for a microsporidian parasite with an estimated genome size of...

02 March 2013 1,000 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

Stacey Human

This is a pretty good kit to use: http://www.lifetechnologies.com/order/catalog/product/AM1626

Sorry, I didnt read the question properly before I answered. I know that we use the SP6 polymerase from GE Healthcare for in vitro transcription in our lab. Here's a link to the Fisher scientific website that may help: https://www.fishersci.ca/searchResults.do?keyword=sp6+polymerase+in+vitro+transcription

You can also see the protocol this lab uses, its free to download: http://www.flyrnai.org/DRSC-PRS.html.

Ok last answer from me, promise! :)

Jamie Bojko

Thanks very much for your help :)