Does anyone have an SOP for dsRNA production in vitro or know where I could find one?

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18S PCR primers for Crustacea - do any exist?

Hey everyone, I was wondering if anyone has tested/published some crustacean focussed 18S primers that provide an amplicon size of around 1kb to use for phylogenetics? Many thanks!

08 September 2018 8,941 3 View

Has anyone managed to install BioLinux onto an external hard drive to use alongside Windows 10? Do you know of a help page?

I need to download BioLinux but down want to wipe my laptop. I have been told I can download onto a USB or external hard drive but I cant find a definitive method that walks me through the...

10 November 2016 4,736 0 View

Bt toxins that target and induce mortality in crustaceans?

Hi, I am currently looking into the development of Bt toxins for crustacean control and have come across zero articles that attempt to develop Bt toxins that target, and induce mortality in,...

03 April 2016 8,815 4 View

Does anyone know how I can acquire some of these amazing amphipods?

Hi there, I am interested in doing some parasitology study on these amazing, giant amphipods. Its a bit of a longshot but if anyone out there is able to get hold of some I would be very interested...

04 May 2015 9,536 3 View

Is anyone working on control methods of vertebrate pests in agriculture? - What methods are allowed to be used for control?

Thank you for looking at this question. I am interested to know what regulations are involved in creating a control plan for vertebrate pests and what differences are in place between these and...

08 September 2013 9,057 4 View

Many invertebrates are agricultural pests but which species are the most studied?

I am interested in control methods used to prevent pest populations causing havok to agricultural industry. In particular I am most interested in biological control methods. If anyone knows any...

04 May 2013 2,535 9 View

The Volga River in Russia: geography, invasive profile and pathogen presence. Is anybody able to provide information?

Future work has directed an interest to The Volga Rover in Russia. Is anyone able to provide me with information on the region and in particular the best time to sample macroinvertebrates? As...

03 April 2013 6,538 6 View

Have any parasitology studies been performed on Gecarcoidea natalis, the red land crab native to Christmas Island?

I hope to look into the parasitology of this species but so far have only come across a parasitic Drosophilid which has been noticed to lay eggs around the secondary antennae of this crab. Are any...

02 March 2013 9,597 2 View

Can anyone recommend a reliable Genome annotation software?

Pending work on annotating a viral genome (~1Mb) and a microsporidian genome (~7.5Mb) I hope to find a reliable genome annotation software which is capable of detecting a variety of genetic...

02 March 2013 9,644 11 View

Genomic Study Costs

Is anyone able to give me a ballpark estimate on the price required to sequence a DNA virus between 0.5-1.0Mb? The same question for a microsporidian parasite with an estimated genome size of...

02 March 2013 859 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Stacey Human

This is a pretty good kit to use: http://www.lifetechnologies.com/order/catalog/product/AM1626

Sorry, I didnt read the question properly before I answered. I know that we use the SP6 polymerase from GE Healthcare for in vitro transcription in our lab. Here's a link to the Fisher scientific website that may help: https://www.fishersci.ca/searchResults.do?keyword=sp6+polymerase+in+vitro+transcription

You can also see the protocol this lab uses, its free to download: http://www.flyrnai.org/DRSC-PRS.html.

Ok last answer from me, promise! :)

Jamie Bojko

Thanks very much for your help :)