18S PCR primers for Crustacea - do any exist?

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Has anyone managed to install BioLinux onto an external hard drive to use alongside Windows 10? Do you know of a help page?

I need to download BioLinux but down want to wipe my laptop. I have been told I can download onto a USB or external hard drive but I cant find a definitive method that walks me through the...

10 November 2016 4,736 0 View

Bt toxins that target and induce mortality in crustaceans?

Hi, I am currently looking into the development of Bt toxins for crustacean control and have come across zero articles that attempt to develop Bt toxins that target, and induce mortality in,...

03 April 2016 8,815 4 View

Does anyone know how I can acquire some of these amazing amphipods?

Hi there, I am interested in doing some parasitology study on these amazing, giant amphipods. Its a bit of a longshot but if anyone out there is able to get hold of some I would be very interested...

04 May 2015 9,536 3 View

Does anyone have an SOP for dsRNA production in vitro or know where I could find one?

I need an SOP for in vitro transcription.

01 February 2014 7,261 4 View

Is anyone working on control methods of vertebrate pests in agriculture? - What methods are allowed to be used for control?

Thank you for looking at this question. I am interested to know what regulations are involved in creating a control plan for vertebrate pests and what differences are in place between these and...

08 September 2013 9,057 4 View

Many invertebrates are agricultural pests but which species are the most studied?

I am interested in control methods used to prevent pest populations causing havok to agricultural industry. In particular I am most interested in biological control methods. If anyone knows any...

04 May 2013 2,535 9 View

The Volga River in Russia: geography, invasive profile and pathogen presence. Is anybody able to provide information?

Future work has directed an interest to The Volga Rover in Russia. Is anyone able to provide me with information on the region and in particular the best time to sample macroinvertebrates? As...

03 April 2013 6,538 6 View

Have any parasitology studies been performed on Gecarcoidea natalis, the red land crab native to Christmas Island?

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02 March 2013 9,597 2 View

Can anyone recommend a reliable Genome annotation software?

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02 March 2013 9,644 11 View

Genomic Study Costs

Is anyone able to give me a ballpark estimate on the price required to sequence a DNA virus between 0.5-1.0Mb? The same question for a microsporidian parasite with an estimated genome size of...

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In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

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02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

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PACYC PCR not working after several attempts. Would anyone have any suggestions?

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02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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01 March 2021 8,544 1 View

How to calculate the annealing temperature of a primer pair?

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01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

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01 March 2021 8,683 4 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

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28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

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28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Jamie Bojko

Thanks for the help!

I have used the Medlin primers but get a low level of amplification due to the size of the amplicon and limited material. I want to avoid cloning if possible and would prefer a nested set I could use internally to the Medlin-amplified sequence.

Jamie

Hiba Riyadh Al-abodi

https://www.researchgate.net/deref/http%3A%2F%2Fwww.tandfonline.com%2Fdoi%2Fpdf%2F10.1080%2F12265071.2000.9647553