Hello Mélanie. In my lab we use RPMI with 5% human serum  for 7-14 days to generate human monocyte-derived macrophages. This serum has M-CSF and GM-CSF ideal proportions to you goal.

Hi Melanie

I think you will find extensive literature comparing Thioglycolate peritoneal macrophages to BMDMs. These populations are very different in their cytokine responses, migration patterns e.t.c. I think its best to look at what most studies in your feild use and as long as you control for your experiments then you can draw concolusions based on that. The other considerations that you might want to make are cost (MCSF can be costly) and time (it takes ~ 7days to get BMDMs in culture).  

Good luck

Thank Julio, I will try to differenciate my human monocytes with human serum.

Simon, actually, I found a lot of papers about peritoneal macrophages and BMDM, but it is always in mouse study. I would like to have human macrophages which can be similar to mouse peritioneal macrophages.

In our studies, we use elicited macrophages in mice and my goal is to demonstrate the same effect in human biology. My first results using M-CSF monocyte-derived macrophages are not so good. I'm asking me that maybe my cells are not enough differenciated and maybe it missed an inflammatory signal to mature the cells.

You can try differentiating with LPS if you are looking at M1 responses.  But select your dose carefully as we have seen increased cell death with LPS. 

You might want to try to differentiate human monocytes in the presence of GM-CSF. They are more pro-inflammatory. 

I just cultured  BM  cells in RPMI medium supplemented with M-CSF (100ng/ml) and I got well differentiated macrophages at day 4,,,,,