I'm looking for a protocol to study the effect of various plasma/serum factors in cell culture. Do Plasma and Serum have different effects? Is there specific storage for Plasma/Serum?
Bonsoir, pour réaliser cette étude il faut cibler le type cellulaire, et théoriquement, le plasma et le sérum n'ont pas les mêmes effets sur le cellules en culture, (sachant que le sérum est débarrassé de facteurs de coagulation y compris le calcium). bon courage
ce sont des cellules musculaires. Avez vous fait ce genre d'expérimentation?
Dear Wiem,
You can expect differences when using plasma versus serum since the serum contains more growth factors such as PDGF, TGF-b, VEGF, b-FGF, HGF, BDNF than plasma. These growth factors are present approximately in the 0.1-50 ng/ml range in serum, in principle. Plasma contains less growth factors but should have IGF-1 at level close to 50 ng/ml. These supplements can be used at a concentration of 5-15% in the basal medium. As Nadjia mentions, serum does not contain coagulation factors including fibrinogen, as well as most of the factors of the complement system. When using plasma it is advisable to add 2-3 U/ml of heparin to avoid eventual clogging of the growth medium in the presence of the cells. Bonne chance, Thierry
Merci Thierry d'avoir répondu à ma question. what's, generally, the difference between serum and plasma (except the groth factors)?
De rien. The total protein content of serum will be a little less as you loose about 2-3 g/L of fibrinogen, then some fibronectin, and traces proteins like factor VIII, von Willebrand proteins, Factor VII, Factor IX etc... Total protein content of plasma can be close to 55-62 g/L and that of serum 50-58 g/L, with variations from donors to donors. You can expect also to have more white blood cells (and of course platelets) in the plasma, these cells being entrapped in the clot formed when serum is prepared. Otherwise albumin, IgG, alpha-1 AT, ransferrin, haptoglobin etc...should be in roughly equivalent amounts in plasma and serum. Cordialement, Thierry
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