Are you using 4PL method to analyse ELISA data?

I'm currently analysing data of Inteleukin measurement with ELISA. Are you using 4PL Method? Which Software are you using? How can you verify if your Standard curve is correct with 4PL method?

09 October 2017 4,890 2 View

What is GitHub??

What GitHub is for? is it like clouds for softwares? Thank you

07 August 2016 7,745 1 View

How to calculate the molarity of 30% hydrogen peroxide?

06 July 2016 6,036 0 View

Does increased messenger RNA level induce increased protein synthesis?

Does increased messenger RNA level induce increased protein synthesis?

10 November 2015 1,701 1 View

Does increased messenger RNA level induce increased protein synthesis?

09 October 2015 3,613 0 View

Any suggestions on Vitamin C Measure and HPO3?

In order to measure Vitamin C, we use metaphosphoric acid (HPO3) to eliminate copper and make the ascorbic acid stable. However, for HPO3 lack, we are looking for another product which have the...

02 March 2015 7,217 6 View

How can we make H3PO4 conversion to HPO3?

01 February 2015 6,960 5 View

Does anyone have an idea about the use of Plasma/serum in cell culture?

I'm looking for a protocol to study the effect of various plasma/serum factors in cell culture. Do Plasma and Serum have different effects? Is there specific storage for Plasma/Serum?

11 December 2014 6,798 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View