Are you using 4PL method to analyse ELISA data?

I'm currently analysing data of Inteleukin measurement with ELISA. Are you using 4PL Method? Which Software are you using? How can you verify if your Standard curve is correct with 4PL method?

09 October 2017 4,890 2 View

What is GitHub??

What GitHub is for? is it like clouds for softwares? Thank you

07 August 2016 7,745 1 View

How to calculate the molarity of 30% hydrogen peroxide?

06 July 2016 6,036 0 View

What does Forest Plot mean??

04 May 2016 661 2 View

Does increased messenger RNA level induce increased protein synthesis?

09 October 2015 3,613 0 View

Any suggestions on Vitamin C Measure and HPO3?

In order to measure Vitamin C, we use metaphosphoric acid (HPO3) to eliminate copper and make the ascorbic acid stable. However, for HPO3 lack, we are looking for another product which have the...

02 March 2015 7,217 6 View

How can we make H3PO4 conversion to HPO3?

01 February 2015 6,960 5 View

Does anyone have an idea about the use of Plasma/serum in cell culture?

I'm looking for a protocol to study the effect of various plasma/serum factors in cell culture. Do Plasma and Serum have different effects? Is there specific storage for Plasma/Serum?

11 December 2014 6,798 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View