Does increased messenger RNA level induce increased protein synthesis?
I'm currently analysing data of Inteleukin measurement with ELISA. Are you using 4PL Method? Which Software are you using? How can you verify if your Standard curve is correct with 4PL method?
09 October 2017 4,890 2 View
What GitHub is for? is it like clouds for softwares? Thank you
07 August 2016 7,745 1 View
06 July 2016 6,036 0 View
04 May 2016 661 2 View
10 November 2015 1,701 1 View
In order to measure Vitamin C, we use metaphosphoric acid (HPO3) to eliminate copper and make the ascorbic acid stable. However, for HPO3 lack, we are looking for another product which have the...
02 March 2015 7,217 6 View
01 February 2015 6,960 5 View
I'm looking for a protocol to study the effect of various plasma/serum factors in cell culture. Do Plasma and Serum have different effects? Is there specific storage for Plasma/Serum?
11 December 2014 6,798 5 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
01 March 2021 8,766 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
