Does increased messenger RNA level induce increased protein synthesis?
I'm currently analysing data of Inteleukin measurement with ELISA. Are you using 4PL Method? Which Software are you using? How can you verify if your Standard curve is correct with 4PL method?
09 October 2017 4,953 2 View
What GitHub is for? is it like clouds for softwares? Thank you
07 August 2016 7,775 1 View
How to calculate the molarity of 30% hydrogen peroxide? Thank you
06 July 2016 6,077 0 View
In Meta-analysis, there's always a "Forest Plot" to estimated results from a studies included in the Flow Chart. Could you please explain more? How can i read the graph? How can i perform it?
04 May 2016 691 2 View
10 November 2015 1,738 1 View
In order to measure Vitamin C, we use metaphosphoric acid (HPO3) to eliminate copper and make the ascorbic acid stable. However, for HPO3 lack, we are looking for another product which have the...
02 March 2015 7,263 6 View
I have to measure the Vitamin C with the Acid Metaphosphoric (HPO3). Unfortunately, I only have Acid Phosphoric (H3PO4). How can i sunthesize HPO3 from H3PO4? NB: I think H20 is the key
01 February 2015 7,010 5 View
I'm looking for a protocol to study the effect of various plasma/serum factors in cell culture. Do Plasma and Serum have different effects? Is there specific storage for Plasma/Serum?
11 December 2014 6,824 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
