Does anyone have advice for designing primers for large, dense CpG island like what we see in the bovine C-MYC gene promoter?

28 March 2018 1 2K Report

DNA bisulfite sequencing primer problems

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Science method

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Is there any researches on the effectiveness of Case Study Approach in Learning Contemporary World?

I would like to evaluate the effectiveness of Case Study Approach on the Achievement of college students in Learning Contemporary World.

07 November 2019 7,458 2 View

Does treated and untreated 96 well plate matter in MIC determination?

I'm working on MIC determination using cell culture coated 96 well plate (NUNC Catalogue no. 167008) and just wonder if this will make any difference with using normal untreated plate? I'm dealing...

08 March 2019 8,398 5 View

How to perform Encapsulation efficiency of liposomal drugs other than centrifugation method?

I have been working on EE studies for my liposomal drug. Size of the particle is around 70-100nm and I measure my drug concentration using UV-Vis method. I have tried centrifuging at ~17,000g for...

26 January 2019 7,088 14 View

What am I doing wrong with regard to fixing/shearing my testis tissue chromatin for ChIP?

Hello all. I am trying to do ChIP-seq with testis tissue, and I have been having a lot of trouble with sorting out my shearing conditions. Essentially, my sheared and unsheared chromatin always...

10 October 2018 3,474 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View