Using RNAscope® 2.5 HD Detection Reagent – RED on FFPE sections
Jarosite is obtained from bioleaching residue and I want to determine if there are increased active sites biogenic jarosite compared to chemical synthetic jarosite.
10 July 2024 8,132 2 View
Scales?
10 July 2024 1,176 2 View
Hi, I would like to gain an insight on the various methods of measuring biomass and growth of Aspergillus oryzae, which you have personally tried and would gladly recommend. Thanks.
08 July 2024 6,057 2 View
Hi, I am trying to measure the area of the caudal fin in zebrafish, to study regeneration. I need to measure the area of the regenerating fin every 3 days after injury. So far, i have been using...
27 June 2024 5,068 1 View
The major causes of post harvest loss of produce in Ghana.
20 June 2024 6,445 5 View
Hi, I want to analyze the protein expression levels of HPV16 E6/E7 in the cervical cancer cell lines SiHa and CaSki after treatment with silencing RNA for this oncogene. Can anyone recommend a...
03 June 2024 9,579 3 View
I'm implanting Alzet pumps in mice with a brain infusion cannula to deliver into the ICV space. I am familiar with the technique that includes removing the pump from the tubing and then using a...
22 May 2024 7,121 3 View
Hi, I am performing optimization calculations in VASP for oxides. After my calculation was finished, I checked the OUTCAR file, and the optimization finished well. But when I checked the other...
29 April 2024 1,506 2 View
Hello, I have data in SPSS from a scale I did, I have 1 variable which is age (separated into 3 categories using values of 1-3), a 2nd variable which is gender (again separated into 3 categories...
19 April 2024 9,983 3 View
We are trying to use the Life Diagnostics Trout HSP70-23 to look at Heat Shock Protein 70 in fish red blood cells to look at physiological changes in response to stressors. I have found very...
08 April 2024 1,134 0 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?
04 August 2024 1,537 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
