We want to stimulate PNT2 or other prostate cells with DHT to stimulate AR dependent gene expression. What is the best way to do that and where can one buy cell culture grade DHT?
See our papers in PubMed. We have described conditions to induce the expression of androgen-inducible IFI16 gene. If it is not known whether PNT2 cells are androgen-responsive and express detectable levels of functional AR, it needs to be done before real experiments.