Has anyone used both milk and BSA as blocking agents/ab diluents at the same time?

Hi, I recall a few years back in a lab I used to work in that I used a 1:1 combination of 5% milk and 5% BSA in TBS-T for blocking and as an ab diluent for western blotting. It seemed to work...

03 April 2019 5,284 3 View

How to calculate sample size a priori in multi-factor experimental design?

Hi there, I am designing an experimental with several groups and wish to reliably determine sample size a priori for each group where I have an effect size set at 0.25 and power of 0.8. I have a...

31 December 2018 9,258 3 View

Does anyone a way to determine post-hoc statistical power from 2 and 3-way ANOVAs?

Hi, I'm dealing with data where I'm testing several 2 and 3-way interactions. However, I also want to be able to determine the statistical power of those that are statistically significant...

03 April 2018 5,952 6 View

What concentrations are suitable for measuring oxidative stress in tissue lysates via spectrophotometry?

I plan to measure total antioxidant status (TAS) and total oxidant status (TOS) in tissue lysates using kits purchased from Rel Assay. I have measured the protein concentration in all my lysates...

01 February 2018 5,881 0 View

Interpreting pairwise contrasts from lsmeans in R?

Hi there, I need assistance with interpreting the outcome of my pairwise comparisons from my datasets. I've been running a glmer mixed models and selecting the best model using the AIC criteria....

06 July 2017 9,277 2 View

Mounting tissue sections in the dark?

05 June 2016 1,281 4 View

BDNF or TRKB as a neurogenic marker?

04 May 2016 6,174 2 View

Use of quantitect QIAGEN primer assays on other RT-PCR systems?

Hi. Do QIAGEN's quantitect primer assays have to be used on their ROTOR-gene system or can they be used on any qPCR machine? Thanks, David

05 June 2015 7,166 1 View

Does anyone have experience of using an Oxford Vibratome Moddel G?

11 December 2014 8,387 2 View

Does anyone have any advice on making slice cultures of adult avian brain?

09 October 2014 7,666 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View