I plan to measure total antioxidant status (TAS) and total oxidant status (TOS) in tissue lysates using kits purchased from Rel Assay. I have measured the protein concentration in all my lysates and plan to use the same concentration for all samples. The kits say they are suitable for use with tissue lysates but don't provide a specific working concentration range.
Therefore I plan to run several dilutions to check my samples are within working range of the assay (comparing with the standards provided). However, I am unsure of what concentration range I should apply. I was thinking a top concentration of 200ug/ml, then 150ug/ml, 100ug/ml, 50ug/ml, 25ug/ml. I have four different tissues for each individual and therefore plan to run the same dilutions for all the tissues too.
I would appreciate if anyone could inform me if they think my dilution range is too low or high, or if I need to change my strategy.
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