David J Walker

12 Questions 11 Answers 0 Followers

Questions related from David J Walker

Has anyone used both milk and BSA as blocking agents/ab diluents at the same time?

Hi, I recall a few years back in a lab I used to work in that I used a 1:1 combination of 5% milk and 5% BSA in TBS-T for blocking and as an ab diluent for western blotting. It seemed to work...

04 April 2019 5,339 3 View

How to calculate sample size a priori in multi-factor experimental design?

Hi there, I am designing an experimental with several groups and wish to reliably determine sample size a priori for each group where I have an effect size set at 0.25 and power of 0.8. I have a...

01 January 2019 9,320 3 View

Does anyone a way to determine post-hoc statistical power from 2 and 3-way ANOVAs?

Hi, I'm dealing with data where I'm testing several 2 and 3-way interactions. However, I also want to be able to determine the statistical power of those that are statistically significant...

04 April 2018 6,017 6 View

What concentrations are suitable for measuring oxidative stress in tissue lysates via spectrophotometry?

I plan to measure total antioxidant status (TAS) and total oxidant status (TOS) in tissue lysates using kits purchased from Rel Assay. I have measured the protein concentration in all my lysates...

02 February 2018 5,936 0 View

Interpreting pairwise contrasts from lsmeans in R?

Hi there, I need assistance with interpreting the outcome of my pairwise comparisons from my datasets. I've been running a glmer mixed models and selecting the best model using the AIC criteria....

07 July 2017 9,326 2 View

Mounting tissue sections in the dark?

Hi there, Yet another IF-related question. I'm going to be dual-labelling free-floating tissue sections with fluorescent secondary abs. After the final wash steps, I will be mounting sections...

06 June 2016 1,355 4 View

BDNF or TRKB as a neurogenic marker?

Hi there, I'm planning some immunofluorescence where I want to dual label either BDNF or TRKB with caspase-3. However, for the species of tissue I'm using, I need to use an antibody that hasn't...

05 May 2016 6,219 2 View

Use of quantitect QIAGEN primer assays on other RT-PCR systems?

Hi. Do QIAGEN's quantitect primer assays have to be used on their ROTOR-gene system or can they be used on any qPCR machine? Thanks, David

06 June 2015 7,228 1 View

Does anyone have experience of using an Oxford Vibratome Moddel G?

I've recently acquired this vibratome which I plan to use to make organotypic slices. I have figured out how it works and I've practiced slicing some 4% agarose blocks (I plan to embed my...

12 December 2014 8,434 2 View

Does anyone have any advice on making slice cultures of adult avian brain?

I plan to perform brain slice cultures from different aged quail (embryonic right up to adulthood) and was wondering if anyone knows of a method, ideally for quail or similar species.

10 October 2014 7,714 5 View

Does anyone have a method for isolating and culturing avian neurons from adult quail/chicken?

I am looking to isolate neurons from different brain regions in adolescent and adult quails to look at cellular senescence and kinases implicated in metabolism. Does anyone have a method for...

10 October 2014 2,173 2 View

Does anyone have any papers detailing a method to isolate and culture neuronal cells from avian hippocampus?

I am looking for papers detailing a method to isolate primary neurons from an avian species and establish them in culture. In particular, I would like an idea of what sort of cytokines/growth...

03 March 2014 3,309 2 View