Hi there,
I'm planning some immunofluorescence where I want to dual label either BDNF or TRKB with caspase-3. However, for the species of tissue I'm using, I need to use an antibody that hasn't been raised in rabbit, as the caspase ab we have is anti-rabbit and validated for our species. As most antibodies for BDNF are anti-rabbit, I was wondering if measuring TRKB expression is as good an indicator of neurogenesis as BDNF, or is this only when the case when BDNF is liganding with TRKB.
Having said that, I have sourced an anti-sheep polyclonal BDNF ab but I need to validate it first but would like some advice on using TRKB from people with more expertise than myself!
Thanks in advance.
Anti-rabbit antibodies are good at detecting neurogenesis.
Use BDNF rather than TRKB .
You could also use other markers for neurogenesis as doublecortine and BRDu. Maybe in this cases the combination of secondary antibodies will suit better your experiment.
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology
- Antibodies