Does anyone Happen to know what BioRad's iProof PCR enzyme is (beyond what's in the catalog description)?

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What is the simplest mathematical model of a mechanical metamaterial that contains rigidity and an external vibration disturbance?

What is the simplest mathematical model of a mechanical metamaterial that contains rigidity and an external vibration disturbance? In my mind, there are mechanical oscillatory systems with...

27 May 2024 1,765 1 View

Any paper discussing influence of wheeler cap resistance on measured efficiency error?

I have a question about estimating the antenna efficiency measurement error when using an improvised Wheeler cap with bad surface resistance?

01 May 2024 8,679 1 View

Fixed or fluidized bed?

Suppose we dry the particles in a fixed bed or fluidized bed. If a particle of bulk material remains inside the apparatus, then the velocity of gas flow around the particle does not exceed the...

24 December 2023 6,987 4 View

Potential baseline drifts down in patch-clamp?

Hi! I do patch-clamp on cardiomyocytes in whole-cell configuration, voltage-clamp mode. Everything went well but suddenly the baseline started to drift down (when the electrode is in the bath,...

22 November 2023 4,376 0 View

De Gruyter reference style, where I can get it?

I am currently preparing a manuscript for Nanotehnology Reviews. Dear sirs, please tell me where I can download De Gruyter reference style for Mendeley? Best regards, Andrey

13 October 2023 6,176 1 View

Is it possible to calculate Molecular Dynamics in Drug Discovery online?

I need to calculate the molecular dynamics of the interaction of quercetin and isorhamnetin with neuraminidase

04 September 2023 3,113 3 View

Can a regenerated cellulose dialysis bag/membrane be used for humidification of air flow?

I need to build a low-cost airflow humidifier which would not have a direct contact between water and the air to be humidified (i.e. not a bubbler or sponge). The "state of the art" on this are...

01 September 2023 4,881 1 View

SMS-7630, can anyone share a consistent RF model?

With 7630 Schottky there are some basic SPICE parameters supplied and s2p for performance demonstrations. I have seen many complains that the provided data is at least inconsistent, and suitable...

21 August 2023 199 0 View

From IV to mediator and mediator to DV are significant. But why the mediation analysis IV-M-DV is not significant ?

Direct relationship between IV-DV is also not significant I calculate using SmartPLS4. Would you help to explain ? Thank you

21 July 2023 1,735 1 View

Hi all! Is there any minimal threshold in DNA concentration, extracted from plasma, as the template for methylation-specific PCR?

"Old world" type of question indeed)), that is to distinguish between methylated or unmethylated CpG loci in DNA from plasma of cancer and non-cancer samples. Unfortunately we have limited access...

09 July 2023 6,416 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Could someone explain the basics of working with enzymes?

I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...

01 August 2024 5,460 5 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

What exactly is it about biotechnology and medical research?

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

28 July 2024 3,798 4 View