What public open software would you recommend for designing HRM primers?

What public open software would you recommend for designing HRM primers?

12 October 2020 3,925 4 View

ISH or immunostaining of GABA neurons?

Hi, Can I reliably label GABA neurons using ISH instead immuno-staining? It is extremely difficult to stain GABA neurons in VLPO and MnPO of hypothalamus by antibody and it seems that ISH is more...

21 September 2020 2,503 4 View

Are there any approved products that can prevent or treat COVID-19 in animals?

10 September 2020 8,643 19 View

Are my birds safe from COVID-19, and is my family safe around my birds?

07 September 2020 4,102 17 View

What tests can be used for a random number generator besides diehard?

What tests can be used for a random number generator besides diehard? Need find tests for my random number generator, based on human-computer interaction.

15 May 2020 7,167 7 View

What is the average depth explored in metal oxide samples through the following characterization techniques: XRD, XPS and Raman spectroscopy?

Obviously, the exact depth evaluated by each characterization technique mentioned (XRD, XPS and Raman spectroscopy) depends on the specific parameters adopted for each analysis. However, in...

15 May 2020 6,418 16 View

How can I add the journal name if your system does not find it in the list?

Hi, I have the published papers (with DOI) in the academic journals that have official ISSN etc. But I can not add and save the names of the journals in my records because your system does not...

26 April 2020 9,394 5 View

What are the storage conditions for phosphines for Staudinger ligation?

Hi all, We would like to purchase 5 mg of phosphine-modified fluorescent dye for site-specific labeling of proteins containing the unnatural aminoacid azide-phenylalanine. We will be labeling...

05 March 2020 3,007 3 View

Metallic materials (more specifically, nickel) present characteristic peaks in Raman spectroscopy analysis?

When evaluating an alumina sample using Raman spectroscopy, characteristic peaks were obtained for certain wavelengths. However, after depositing a thin nickel film via plasma on the surface of...

24 January 2020 8,954 3 View

Does anybody have PDF version of MOE manual?

I will highly appreciate if you can share one for program version 2014 or higher.

28 May 2019 5,615 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View