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Questions related from Nathan Ponzar
I have been doing a lot of thermofluor assays for determining binding affinities. This necessitates getting the raw fluorescence values organized into a different column for each sample....
31 July 2021 911 2 View
Say you have an enzyme with 3 ligands: a substrate, a cofactor, and an inhibitor. They all interact and effect one another. If one was to conduct a 3-dimensional titration of these ligands, are...
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I think this is a valid approach to determining Ki of competitive and non-competitive inhibition for mixed model inhibitors, but I would appreciate feedback. On this graph, the Y-values are...
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Hello, I have some data that looks like the line on the right, but I'm not sure what function to use. It seems like an exponential added to a line, or something of the like. I think it may be the...
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It is my understanding that single-turnover reactions isolate the chemical step, as opposed to being comprised of the chemical step + product dissociation as in kcat. Here's my issue though....
14 November 2020 2,621 7 View
I express a recombinant protein in E. coli that requires Mg2+ and ATP to remove bound chaperones and activate the enzyme, but also requires TCEP. I currently achieve active protein, but with very...
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