Hello Ivan

Have you tried other fingerprint methods ?

RAPD may not have a enough sensitivity.

Good luck!!

Marcia

UEL.

Hi Marcia,

Thank you very much for your feedback!

The doses are pretty high and we are using primers and a similar protocol that worked for cyanobacteria at similar doses:

Mickael Baqué, Cyprien Verseux, Elke Rabbow, Jean-Pierre Paul de Vera,  Daniela Billi (2014) Detection of Macromolecules in Desert Cyanobacteria Mixed with a Lunar Mineral Analogue After Space Simulations Orig Life Evol Biosph (2014) 44:209–221. DOI 10.1007/s11084-014-9367-4

In our experiments, the primers described by Baqué et al. (2014) are working for Escherichia coli but not for Geodermatophilus sp. So I was hoping someone had RAPD primers optimized for Geodermatophilus spp, which has high GC content.

In any case we are also using a thymine-dimer detection protocol, which is more sensitive and quantitative, and hopefully will give us good preliminary data on DNA damage and repair in Geodermatophilus sp.

Best wishes!

Ivan