We have been trying to get by IHC labeling of F4/80 positive cells in our lungs sample from mice (macrophages)
The best we could get was a weird labeling in red.
We do not label the cells as the labeling is smaller than the nucleus.
It seems that our Ab is being trapped in some particular unknown structures...
So right now, I am not trusting this can of picture to say that I'm seeing F4/80 cells.
Any thoughts?
Here the following info to understand the pics:
it is confocal microscopy: depth of the plan: 0,125 um.
blue is DAPI
green is an FITC-labelled Ab
The 2 pics show you different fields.
F4/80 clone AB used: BM8
Thanks in advance for your help.
Alexis
We've used Serotec's F4/80 rat mAb (product code MCA497R) successfully in FFPE murine tissues (IHC)
Hi Daniel,
Thank you so much for your contribution.
We tried your Ab and it's finally working!!
thanks again
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