How to get a nice CD107a signal in mouse by flow cytometry?

I tried to monitored CD107a labelling in mouse NK cells after infection (compared to uninfected) but I'm unhappy with the plot I got. CD107a Ab clone 1D4B rat IgG2a,k from BD Bioscience cell are...

09 October 2018 6,376 3 View

Any protocol for RT-PCR on fixed cells purified by FACS?

We are having trouble looking up at the cellular source of our interleukin by regular intracellular staining by flow cytometry. We'd like to try to detect the mRNA in our cell of interest from...

05 June 2016 5,261 3 View

Can anyone provide incite into a spontaneous release of IFN-gamma by NK cell lines?

Hi, A year ago, I did some ELISA experiments in the medium of cell culture of NK92 and got low IFN-gamma release (around 50pg/mL). It was nothing compared to my stimulated conditions where I got...

06 July 2014 1,205 7 View

Which is the best way to deplete alveolar macrophages in mice?

06 July 2014 3,080 2 View

Anti-CD11c mouse Ab working in paraffin-embedded sections?

We have been trying to get by IHC labeling of CD11c positive cells in our lungs and spleen sample from mice. The best we could get was a weird labeling in red. We do not label the cells as the...

31 December 2013 3,914 15 View

Mice general shape scoring scale?

Hello all, I'm working in the field of infectious diseases and I'm using mice models of infection. I saw a paper some times ago in which the authors using a scoring system in order to measure the...

31 December 2013 8,527 2 View

Does anybody know a working antibody anti F4/80 working in paraffin-embedded mouse tissue?

We have been trying to get by IHC labeling of F4/80 positive cells in our lungs sample from mice (macrophages) The best we could get was a weird labeling in red. We do not label the cells as the...

31 December 2013 7,094 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View