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Questions related from Alexis Broquet
I tried to monitored CD107a labelling in mouse NK cells after infection (compared to uninfected) but I'm unhappy with the plot I got. CD107a Ab clone 1D4B rat IgG2a,k from BD Bioscience cell are...
10 October 2018 6,429 3 View
We are having trouble looking up at the cellular source of our interleukin by regular intracellular staining by flow cytometry. We'd like to try to detect the mRNA in our cell of interest from...
06 June 2016 5,322 3 View
We would like to deplete alveolar macrophages in mice and we cannot find suitable antibodies (F4/80?). We will be using random Swiss mice (non isogenic). The only way I found is by using...
07 July 2014 3,141 2 View
Hi, A year ago, I did some ELISA experiments in the medium of cell culture of NK92 and got low IFN-gamma release (around 50pg/mL). It was nothing compared to my stimulated conditions where I got...
07 July 2014 1,270 7 View
Hello all, I'm working in the field of infectious diseases and I'm using mice models of infection. I saw a paper some times ago in which the authors using a scoring system in order to measure the...
01 January 2014 8,590 2 View
We have been trying to get by IHC labeling of CD11c positive cells in our lungs and spleen sample from mice. The best we could get was a weird labeling in red. We do not label the cells as the...
01 January 2014 3,979 15 View
We have been trying to get by IHC labeling of F4/80 positive cells in our lungs sample from mice (macrophages) The best we could get was a weird labeling in red. We do not label the cells as the...
01 January 2014 7,156 2 View
I checked on pubmed and on the biocompare.com website and found no antibodies or related studies so far.
12 December 2012 9,258 3 View
